| EERα

Estrogen-related receptor alpha (ERRα), also known as NR3B1 (nuclear receptor subfamily 3, group B, member 1), is a nuclear receptor that is encoded by the ESRRA (Estrogen Related Receptor Alpha) gene.[5][6] ERRα was originally cloned by DNA sequence homology to the estrogen receptor alpha (ERα, NR3A1),but subsequent ligand binding and reporter-gene transfection experiments demonstrated that estrogens did not regulate ERRα.Currently, ERRα is considered an orphan nuclear receptor.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.08	1.31	0.75	0.5	0.33	0.25	0.21	0.09
Corrected OD	1.99	1.22	0.66	0.41	0.24	0.16	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat EERα spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-107	105

 

| Linearity

Samples were spiked with high concentrations of Rat EERα and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-114
	Average (%)	94	98	107